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Histone H3(tri methyl K9) Monoclonal antibody

Catalog No. : EM1109

Specification Price
50µl $148
100µl $248

Overview

Product name: Histone H3(tri methyl K9) Monoclonal antibody
Source: Mouse
Applications: WB, IHC
Species reactivity: Human,Rat,Mouse
Recommended dilutions: WB 1:1,000-3,000 IP 1:200  IHC 1:200-500
Immunogen: Synthetic Peptide
Specificity: Human
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality: Monoclonal
Isotype: IgG1
Concentration: 1mg/ml
Observed band: 15kDa
GeneID(Human): 8290
Human Swiss-Prot No.: P68431
Cellular localization: Nucleus,Chromosome
Alternative Names: H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody
Background: Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
  • Western blot analysis of Hela with EM1109 diluted at 1) 1:2,000 2) 1:5,000

  • Western blot analysis of 1) Rat Testis Tissue, 2) Raw264.7 with EM1109 diluted at 1:2000.

  • 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1109 1:1,000 Secondary antibody: Goat anti-Mouse IgG(H+L) (S001) 1:10,000

  • Immunohistochemical analysis of paraffin-embedded Human Clolon using  Histone H3(tri methyl K9)(EM1109)Mouse mAb diluted at 1:500.