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Vimentin Mouse mAb

Catalog No. : EM1159

Specification Price
50µl $148
100µl $248


Product name: Vimentin Mouse mAb
Source: Mouse
Applications: WB, IHC, IF
Species reactivity: Human,Rat,Mouse
Recommended dilutions: WB 1:1,000-3,000 IF 1:100-200 IHC 1:200-500
Immunogen: Recombinant Protein
Specificity: Human
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality: Monoclonal
Isotype: IgG1
Concentration: 1mg/ml
Observed band: 50-57kDa
GeneID(Human): 7431
Human Swiss-Prot No.: P08670
Cellular localization: Cytoplasm
Alternative Names: FLJ36605 antibody, VIM antibody, VIME antibody
Background: The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules.Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells.
  • Western blot analysis of 1) Hela, 2)Mouse Brain Tissue , 3) Rat Brain tissue with EM1159 diluted at 1:2,000.

  • IF analysis of Hela with EM1159(Left) and DAPI (Right) diluted at 1:100.

  • Immunohistochemical analysis of paraffin-embedded human breast caricnoma using Vimentin (EM1159)Mouse mAb diluted at 1:500.

  • Western blot analysis of extracts from MCF-7 using Vimentin diluted at 1:3,000