COX IV Mouse mAb
Catalog No. : EM1032
|Product name：||COX IV Mouse mAb|
|Recommended dilutions：||WB 1:1,000-3,000 IF 1:100-200|
|Storage：||PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.|
|Human Swiss-Prot No.：||P13073|
|Cellular localization：||Membrane,Mitochondrion,Mitochondrion inner membrane|
|Alternative Names：||COX 4, Cox4a, COX4B, COXIV, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial, MGC105470, dj857M17.2, Cytochrome c oxidase subunit IV|
|Background：||The enzyme cytochrome c oxidase or Complex IV, EC 220.127.116.11) is a large transmembrane protein complex found in bacteria and the mitochondrion.It is the last enzyme in the respiratory electron transport chain of mitochondria (or bacteria) located in the mitochondrial (or bacterial) membrane. It receives an electron from each of four cytochrome c molecules, and transfers them to one oxygen molecule, converting molecular oxygen to two molecules of water. In the process, it binds four protons from the inner aqueous phase to make water, and in addition translocates four protons across the membrane, helping to establish a transmembrane difference of proton electrochemical potential that the ATP synthase then uses to synthesize ATP.|
Western blot analysis of Hela with COX IV Mouse mAb diluted at 1）1:2,000 2）1:5,000
Western blot analysis of 1) HepG2, 2) 293T, 3) Rat Heart, 4) Mouse Heart, 5) Mouse Muscle tissue with COX IV Mouse mAb) diluted at 1:3,000.
Western blot analysis of extracts from Mouse Kidney using COX IV diluted at 1:5,000.
IF analysis of Hela with EM1032(Left) and DAPI (Right) diluted at 1:100.