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Peptide Service


(4-25AA) This price is the unit price of per AA(US $)
Amount Crude Desalted Purity
>70% >75% >80% >85% >90% >95% >98%
1-4 mg $3.5 $6.0 $8.0 $8.3 $8.6 $9.0 $9.5 $10.5 $13.0
5-9 mg $4.0 $6.3 $8.5 $8.6 $9.0 $10.0 $10.5 $11.0 $14.0
10-14 mg $4.3 $6.8 $9.0 $9.2 $10.0 $11.5 $12.0 $12.5 $16.0
15-19 mg $4.6 $7.2 $10.0 $10.5 $11.0 $12.5 $13.5 $14.5 $18.0
20-24 mg $5.0 $7.6 $10.5 $11.5 $12.5 $13.0 $15.0 $16.5 $20.0
25-29 mg $5.3 $8.0 $11.0 $12.5 $13.0 $15.0 $16.0 $17.5 $22.0
30-39 mg $5.8 $8.3 $12.0 $13.5 $14.5 $16.0 $17.0 $19.5 $24.0
40-49 mg $6.3 $8.6 $12.5 $15.0 $15.5 $17.5 $18.5 $20.5 $26.0
50-59 mg $7.0 $9.0 $13.5 $16.0 $17.0 $18.5 $19.5 $21.5 $29.0
60-79 mg $7.5 $10.5 $15.0 $18.0 $20.0 $21.0 $22.0 $24.5 $31.0
80-100 mg $8.5 $11.5 $17.0 $19.0 $22.0 $23.5 $25.0 $27.0 $37.0
200 mg $11.5 $15.5 $21.0 $24.0 $28.0 $30.0 $31.0 $37.0 $46.0
300 mg $16.0 $20.0 $27.0 $33.0 $35.0 $41.0 $43.0 $51.0 $64.0
500 mg $21.0 $25.0 $34.0 $42.0 $48.0 $51.0 $54.0 $63.0 $79.0
1000 mg $29.0 $37.0 $51.0 $62.0 $67.0 $77.0 $80.0 $86.0 $118.0


* The minimum charge per peptide is $125.00.
* Please contact us the price for peptides below 4 amino acids.
* Prices of 25 amino acids or above, grams or above, and special polypeptides are subject to inquiry.

Advantages


* Up to 200aa peptide sequences can be synthesized
* Variety of peptide modification services
* Over 95% of synthesis success rate
* Fastest peptide synthesis speed in the industry
* All peptides are analyzed by MS and HPLC


Applications


* Antibody epitope mapping
* Monoclonal and polyclonal antibody production
* Non-quantitative enzyme-substrate studies
* Biological effects of defined peptides
* Blocking and competition assays of proteases
* New techniques for separation by chromatography/electrophoresis
* Immunization and quantitative receptor-ligand interactions
* Antibody-antigen interactions
* ELISA standards for measuring titers of antibodies
* Epitope mapping Structure, dynamics, and folding of peptides and proteins via NMR


PolyPeptide preservation


* Generally long-term preservation of polypeptides need to avoid light preservation, and should be stored below -20 degrees Celsius, short-term can be stored at 4℃. It can be transported at room temperature for short periods of time. Polypeptides are very stable at -20℃, especially when they are lyophilized and stored in a desiccator. Before they are exposed to air, lyophilized polypeptides can be released at room temperature for naturally heating up, which reduces the effect of humidity effect. When lyophilizing is not possible, the best way is to store it in small amount of working samples.

* For polypeptides containing Cys, Met or Trp, deoxidation buffers are essential for their dissolution, as the polypeptides are prone to air oxidation, and nitrogen or argon protection reduces oxidation prior to sealing. Polypeptides containing Gln or Asn are more susceptible to degradation and all of these peptides have a shorter shelf life than simple peptides that do not contain these problematic peptides.

Polypeptide dissolution


Dissolving a polypeptide is a very complex process, and it is generally difficult to determine a suitable solvent quickly. Usually take a little for a preliminary test, and do not dissolve completely before determining the proper solvent.
The following methods can help you choose the right solvent:
* Determine the charge specificity of the polypeptide, set the acidic amino acid Asp(D), Glu(E) and C-terminal COOH to -1; basic amino acid Lys(K), Arg(R), His(H) and N-terminus NH2 is +1 and the charge of the other amino acids is zero. Calculate the net charge number.
* If the net charge number is >0, the polypeptide is alkaline and dissolved in water; i f the peptide does not dissolve, add 10% acetic acid dropwise with vortexing in between. The peptide solution can also be warmed slightly. Longer peptides (20+ amino acids) with a small overall net charge might require the addition of a stronger acid. Trifluoroacetic acid (TFA 10-50ul) is often used to solubilize peptides but it is not cell-friendly and thus is used only if acetic acid fails to help solubilize the peptide. After the addition of TFA, the peptide should be diluted to approximately 1ml with deionized water.
* If the net charge number is <0, the peptide is acidic and dissolved in water; If the peptide does not dissolve, add ammonium hydroxide (NH4OH 10-50ul) and once in solution, dilute the peptide to approximately 1ml with deionized water. Note: Caution must be used, however, with peptides that contain cysteine (C), as the used of alkaline pH can cause disulfide bond formation.
* If the net charge number = 0, the polypeptide is neutral and generally needs to be dissolved with an organic solvent such as acetonitrile, methanol or isopropanol, DMSO and the like. It has also been suggested that urea is required to dissolve highly hydrophobic polypeptides.